The saliva collection process allows researchers to obtain DNA samples of subjects and parents for genetic testing. Before collection can take place, patient families meet with a research coordinator to review an informed consent form and receive instructions about the saliva collection process. Next, they are provided with collection kits and asked to salivate into the collection tube. Saliva donors may not eat, drink, chew gum or smoke 30 minutes prior to providing a sample.
Saliva Collection Instructions
- Remove the tube from the clamshell and salivate until reaching the fill line.
- Close the funnel lid by pressing down firmly until the lid snaps securely in place.
- While keeping the tube in an upright position, unscrew the funnel from the tube.
- Use the small cap provided to close the tube securely before shaking the tube for at least five seconds.
- Place the tube into the biohazard bag provided and complete the included saliva information form.
- Place the sample bag and completed information form in the provided box before sealing. Pre-paid postage and address are provided, simply drop in any mailbox.
Upon arrival at the CBTTC Operations Center, the saliva sample will be processed and inventoried by a research technician. Saliva samples will be available for use by researchers to discover new treatments and therapies for brain tumor patients.
For additional collection instructions, please click here: DNA Genotek Collection Instructions
Saliva Stimulation Tips
If having trouble providing the required amount of saliva due to dry-mouth, you can try the following:
- Add a pinch of sugar/sweetener or squirt of lemon juice to the tip of the tongue as it helps to stimulate salivation. *Only a small amount is needed; just enough for the person to taste it.
- Rubbing their cheeks right behind the jaw is another option for physically stimulating salivation.
Post Collection Storage
Once collected, Oragene samples are stable at ambient temperatures for 5 years, or indefinitely at -20C or below. It is NOT recommend to refrigerate samples (less than 4ºC/40ºF)